Course information | BBT21113 Instrumentation, Biosafety & Quality Management (MB120,121,122,123,124)

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BBT21113 Instrumentation, Biosafety & Quality Management (MB120,121,122,123,124)

(MB120,121,122,123,124)

Coordinator: Wong Nyet Kui
Coordinator: CHIEW YEN WONG
Coordinator: HUI YENG YEANNIE YAP

1. Overview

Getting to know

Module Learning Outcomes

Content Synopsis

Student Learning Time

Module Guide - BBT21113 Instrumentation, Biosafety &amp; Quality Management

Guided Reading-Microarray Technology

2. Reference

3. Assessment

Mock MCQ exam

Mock SAQ exam

Topic 1 - Electrophoresis I (Lecture – 1 hr)

Electrophoresis 1

Electrophoresis 1 (Recording)

Topic 2 - Electrophoresis II (Lecture – 1 hr)

Electrophoresis 2

Electrophoresis 2 (Recording)

Topic 3 -Tutorial 1: electrophoresis (1 hr)

Electrophoresis Tutorial

Tutorial Electrophoresis (Recording)

Topic 4 - Polymerase Chain Reaction and thermal cycler I (Lecture – 1 hr)

Lecture notes PCR 1

Polymerase chain reaction and thermal cycler 1 - Voice

Video - Basic PCR process

Video - how PCR works

Video - conventional PCR setting up

Video - RT PCR (1 step and 2 steps)

Topic 5 - Polymerase Chain Reaction and thermal cycler II (Lecture – 1 hr)

PCR and Thermal cycler 2 Voice

Lecture notes PCR 2

Video - Differences between PCR, qPCR, dPCR

Video - quantitative PCR

Topic 6 - Tutorial 2: PCR and RT-PCR (1 hr)

Tutorial PCR MB123 student copy

Tutorial file for students to work on

Topic 7- DNA sequencer and protein sequencer (Lecture – 1 hr)

DNA sequencer and protein sequencer

Topic 8 - The chemistry of microarray (Guided reading)

Topic 9 - Oligonucleotide / peptide synthesis and microarrayer (Lecture – 1 hr)

Oligonucleotide / peptide synthesis and microarrayer

Topic 10 - Comparative study of microarray usefulness (Guided reading II)

Topic 11 - Tutorial 3: DNA and protein sequencer (1hr)

Tutorial Questions

Topic 12 - Principle, Instrumentation and control system of centrifuges (Lecture – 1 hr)

Centrifuge 1

Centrifuge 1 Voice Over

Topic 13 - Analytical ultracentrifuge and fractionation of cells (Lecture -1 hr)

Centrifuge 2

Centrifuge 2 Voice Over

Topic 14 - Microscopy I (Lecture – 1 hr)

Microscopy I

Topic 15 - Microscopy II (Lecture – 1 hr)

Microscopy II

Topic 16 - Workshop 1 – electrophoresis, centrifuge, ultracentrifuge (3 hr)

Electrophoresis Tutorial

Reference Article

Workshop 1 Instruction

Topic 17 -Workshop 2 - laboratory equipments (eg. Fluorescent microscope and flow cytometer) (3 hour)

Topic 18 - Workshop 3- Workshop 3: PCR and RT- PCR(3 hr)

workshop PCR student copy

PCR Workshop

MB124 PCR workshop submission

Topic 19 - Optical systems used in spectroscopy (Lecture- 1 hr)

Lecture materials (pdf)

Lecture materials (pptx)

Topic 20 - Visible and Ultraviolet molecular spectroscopy (Lecture – 1 hr)

Lecture materials (pptx)

Lecture materials (pdf)

Topic 21- Operational principle of biosensor and flow cytometry (Lecture – 1 hr)

Biosensor and flow cytometry

Biosensor and flow cytometry audio

Topic 22 - Biosafety I (Lecture – 1 hr)

Lecture materials (pptx)

Lecture materials (pdf)

Topic 23 - Biosafety II (Lecture – 1 hr)

chem-compatibility-chart

chemical storage 9 compatible stirage group system

Lecture materials (pdf)

Lecture materials (pptx)

Topic 24 - Biosafety III (Lecture – 1 hr)

Module Guide - BBT21113 Instrumentation, Biosafety & Quality Management