Course information | BBT21113 Instrumentation, Biosafety & Quality Management (MB125)

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BBT21113 Instrumentation, Biosafety & Quality Management (MB125)

(MB120,121,122,123,124)

Coordinator: Wong Nyet Kui
Coordinator: CHIEW YEN WONG
Coordinator: HUI YENG YEANNIE YAP

1. Overview

Getting to know

Module Learning Outcomes

Content Synopsis

Student Learning Time

Module Guide - BBT21113 Instrumentation, Biosafety &amp; Quality Management

2. Reference

3. Assessment

Mock MCQ exam

Mock SAQ exam

Topic 1 - Polymerase Chain Reaction and thermal cycler I (Lecture – 1 hr)

Video - how PCR works

Video - conventional PCR setting up

Video - RT PCR (1 step and 2 steps)

Topic 2 - Polymerase Chain Reaction and thermal cycler II (Lecture – 1 hr)

PCR and Thermal cycler 2 Voice

Lecture notes PCR 2

Video - Differences between PCR, qPCR, dPCR

Video - quantitative PCR

Topic 3 - Tutorial 1: PCR and RT-PCR (1 hr)

Tutorial PCR MB123 student copy

Tutorial file for students to work on

Topic 4 - Workshop 1: PCR and RT- PCR (3 hr)

workshop PCR student copy

PCR Workshop

MB124 PCR workshop submission

Topic 5 - Electrophoresis (Lecture – 1 hr)

Topic 6 - Advanced techniques in protein analysis (Lecture – 1 hr)

Topic 7 - Tutorial 2: Electrophoresis (1 hr)

Topic 8 - Principle, Instrumentation and control system of centrifuges (Lecture – 1 hr)

Centrifuge 1

Centrifuge 1 Voice Over

Topic 9 - Analytical ultracentrifuge and fractionation of cells (Lecture -1 hr)

Centrifuge 2

Centrifuge 2 Voice Over

Topic 10 - Workshop 2: Electrophoresis, centrifuge, ultracentrifuge (3 hr)

Electrophoresis Tutorial

Reference Article

Workshop 1 Instruction

Topic 11 - DNA sequencer and protein sequencer (Lecture – 1 hr)

DNA sequencer and protein sequencer

Topic 12 - The chemistry of microarray (Guided reading)

Guided reading I &amp; II - Student handout

Topic 13 - Oligonucleotide/peptide synthesis and microarrayer (Lecture – 1 hr)

Oligonucleotide / peptide synthesis and microarrayer

Topic 14 - Comparative study of microarray usefulness (Guided reading II)

Guided reading I &amp; II - Student handout (copy)

Topic 15 - Tutorial 3: DNA and protein sequencer, microarrays (1hr)

Tutorial Questions

Topic 16 - Spectroscopy (Lecture- 1 hr)

Lecture materials (pptx)

Lecture materials (pdf)

Lecture materials (pdf)

Lecture materials (pptx)

Topic 17 - Workshop 3: Spectrophotometers and microarray (3 hr)

Spectrophotometers

Topic 18 - Operational principle of biosensor and flow cytometry (Lecture – 1 hr)

Biosensor and flow cytometry

Biosensor and flow cytometry audio

Topic 19 - Microscopy I (Lecture – 1 hr)

Microscopy I

Topic 20 - Microscopy II (Lecture – 1 hr)

Microscopy II

Topic 21 - Workshop 4:Microscopy (3 hr)

Topic 22 - Biosafety (Lecture – 1 hr)

Lecture materials (pptx)

Lecture materials (pdf)

Topic 23 - Chemical safety (Lecture – 1 hr)

chem-compatibility-chart

chemical storage 9 compatible stirage group system

Lecture materials (pdf)

Lecture materials (pptx)

Topic 24 - Radiation safety (Lecture – 1 hr)

7813788-Clean-Room-Requirement

Chp16-Disciplines

clean room operations

Guided Reading-clean room

read chap 21 23 25 26

Lecture materials (pptx)

Lecture materials (pdf)

Module Guide - BBT21113 Instrumentation, Biosafety & Quality Management

Guided reading I & II - Student handout

Guided reading I & II - Student handout (copy)